Research Associate I-III IN VITRO

Role Summary

The IN VITRO Research Associate will perform research and/or development in collaboration with others for projects. Makes detailed observations, analyzes data and interprets results. Investigates, creates and develops new methods and technologies for project advancement. Location: San Diego, California.

Responsibilities

• Generate immunophenotyping and immune cell functional data using techniques such as multicolor flow cytometry, ELISA, and xCELLigence RTCA.
• Isolate protein, DNA, and RNA from cell cultures and tissues; perform gene and protein analysis using qPCR and Western blot.
• Maintain primary and established cell lines to support in vivo studies.
• Assist with general cell culture for the production of oncolytic viruses, including herpes simplex virus, vaccinia virus, and/or adenovirus.
• Contribute to the generation of recombinant therapeutic viruses.
• Support the in vivo team as needed, including animal handling and sample collection.
• Analyze and organize experimental data with strong attention to detail.
• Assist with laboratory maintenance, inventory, and cleaning tasks.
• Adhere to EH&S guidelines, participate in mandatory safety training, and follow SOPs to ensure study integrity and compliance.
• Perform other duties as assigned.

Qualifications

• Required: 2+ years of experience in a biotechnology company with broad knowledge and experience in cell and molecular biology methods.
• Preferred: Immuno-Oncology field experience.
• Required: Experience with mammalian cell culture.
• Required: Experience in immune cell-based assays (ELISA, MSD, cytotoxicity assays).
• Preferred: Strong hands-on experience in acquiring and analyzing flow cytometry data (multi-parameter flow cytometry, intracellular, extracellular staining, FlowJo analysis).
• Preferred: Experience with mouse handling, including restraint, injection (IP, IV, SC), and tissue collection.
• Preferred: Experience in a range of in vitro cellular/molecular biology techniques (cell culture, cloning, qPCR, Western blot, immunohistochemistry, microscopy).
• Required: Excellent data organization, analysis, and troubleshooting skills using electronic lab notebook.
• Required: Dedication to working in a team-oriented environment to contribute to shared project.
• Required: Strong work ethic, self-motivation, and ability to multi-task in a fast-paced work environment.
• Required: Strong knowledge of MS Word, Outlook, PowerPoint, Excel, GraphPad Prism, FlowJo.

Education

• B.S. or higher in life science, or related field