TEMP - Associate Scientist / Scientist, Neuroimmunology

Role Summary

TEMP - Associate Scientist / Scientist, Neuroimmunology. Contribute to assessment of novel therapeutic approaches to target autoimmunity and inflammation in neurodegenerative and movement disorders. Design, perform, and validate key in vitro cell-based functional assays; study mechanism of action of drug candidates; present data to cross-functional teams.

Responsibilities

• Designing and performing experiments to support scientific activities and project goals
• Separation of human PBMC from whole blood or leukopak, and isolation of primary human leukocyte populations for downstream assays
• Maintain cell lines and primary cell cultures
• Perform immunological assays including lymphocyte proliferation, activation, cytokine secretion, and differentiation
• Perform basic molecular biology assays including mRNA isolation, Taqman qPCR
• Flow cytometric analysis of immune cell subsets and analysis of data
• Perform immunoassays (MSD, ELISA, etc) for analysis of soluble proteins in cell supernatants/murine plasma
• Maintains and updates SOPs and electronic lab notebook, and writes data summaries for experiments conducted
• Present data and findings to cross-functional project teams
• Provides training and/or supervision to junior staff, as needed
• Other duties as assigned

Qualifications

• BS/BA degree and 4+ years of relevant lab experience, OR
• MS/MA degree and 2+ years of relevant experience, immunology background preferred
• Robust experience with whole blood, PBMC, and immune cell-based in vitro assays
• Expertise in cell culture and aseptic technique, involving both immortalized cell lines and primary human/murine cells
• Experience in immunoassays (ELISA, MSD, Western Blot, etc) and molecular biology techniques (mRNA isolation, qPCR)
• Experience with multicolor flow cytometric analysis is a must to have
• Able to explain the process behind data generated and the implications of the results
• Strong knowledge of one or more scientific disciplines, especially immunology
• Strong knowledge of scientific principles, methods, and techniques, and demonstrated ability to work with a variety of laboratory equipment/tools
• Excellent communication, problem-solving, and analytical thinking skills
• Ability to work as part of a cross-functional team, and present data and concepts clearly and concisely
• Demonstrated ability to meet multiple deadlines with a high degree of accuracy and efficiency, and experience prioritizing multiple projects is a plus

Education

• As listed in Qualifications