Senior Scientist, Mass Spectrometry

Role Summary

We’re looking for a highly motivated and experienced bioanalytical scientist to advance mass spectrometry applications from discovery to early-stage development. The Senior Scientist, Mass Spectrometry will apply broad mass spectrometry expertise to biomarker discovery and targeted protein quantitation, and will help characterize protein therapeutics by MS. The role includes developing LC-MS/MS assays, participating in unbiased proteomic biomarker discovery, and collaborating to expand capabilities into metabolomics, lipidomics, and related technologies. This position is based in Bridgewater, NJ and requires full-time, in-person presence.

Responsibilities

• Independently develop LC-MS/MS (i.e., Sciex 7500+) assays for protein target quantitation by robust sample preparation (i.e., protein extraction, proteolysis, etc.), assignment of appropriate signature peptide, establishment of analytical figures-of-merit (i.e., linearity, sensitivity, etc.), and method qualification in representative biological matrices.
• Perform unbiased proteomic experiments for biomarker discovery by employing proteomic workflows, including appropriate experimental design, reproducible sample preparation, high resolution mass spectrometry data acquisition (Thermo Astral, Eclipse, Excedion), bioinformatic data analysis (i.e., ProteomeDiscoverer, MaxQuant, PEAKS, etc.), and results interpretation.
• Expand upon current sample preparation workflows to include affinity-enrichment and offline fractionation approaches for identification and characterization of low-abundance protein analytes.
• Identify and/or employ complementary bioanalytical techniques (e.g., ELISA, MSD, Western-blot) for better understanding of biological results while working closely with internal SMEs for executing experiments of most value.
• Contribute to the routine analysis of therapeutic protein candidates, including peptide mapping, intact/native protein analysis, data acquisition and reporting to provide analytical support of various research projects.
• Assist the group in lab maintenance and housecleaning, ensuring optimum instrument operation and readiness (calibration, QA/QC), stocking of reagents/supplies/chemicals, proper waste disposal, sample inventory and CTU management, and strict adherence to safety protocols.

Qualifications

• Required: Bachelor’s degree in Chemistry, Analytical Chemistry, Biochemistry or a related scientific discipline; strong focus on proteomic analysis using HRMS and QQQ mass spectrometry platforms.
• Required: 5+ years of hands-on experience in applying advanced mass spectrometry techniques for both unbiased protein biomarker discovery and targeted protein quantitation by LC-MS/MS.
• Required: Experience with broad sample preparation methodologies, automation, and instrumentation (Thermo, Sciex).
• Required: Ability to create and perform SRM/MRM experiments on QQQ instruments.
• Required: Experience with LC-MS operation, maintenance, and troubleshooting.
• Required: Strong knowledge of ligand-binding assay development, application, and translation.
• Required: Excellent written and verbal communication skills.
• Required: Strong work ethic, self-motivation, and a collaborative spirit.
• Required: Experience with documentation of scientific data, scientific writing, and presentation of research findings.
• Required: Strict adherence to environmental, health, and safety guidelines.
• Preferred: Strong knowledge of chromatographic separation (e.g., SEC, SCX, HpH RP) highly desired.
• Preferred: Knowledge of electrophoretic (e.g., CE, SDS-PAGE, IEF) and biophysical interaction (e.g., SPR, BLI) a plus.
• Preferred: Experience with metabolomic and lipidomic analysis a plus.
• Preferred: Experience with Top-down and native MS a plus.

Education

• Bachelor’s degree in Chemistry, Analytical Chemistry, Biochemistry or a related scientific discipline.

Additional Requirements

• Travel: Minimal travel expected.