Senior Associate Scientist, Ligand Discovery
Alnylam Pharmaceuticals
June 26, 2026
On-site
Cambridge, MA
Clinical Research and Development
Responsibilities:
- Contribute to the design and execution of ligand selection campaigns using display technologies (e.g., phage/yeast display, MACS/FACS) against diverse membrane proteins.
- Characterize ligand-receptor interactions using biochemical techniques and cell-based assays (ELISA, BLI/SPR, flow cytometry, epitope binning).
- Support library construction and hit sequence recovery using molecular biology techniques (PCR, cloning, plasmid purification).
- Assist with preparation and characterization of soluble and membrane-embedded protein antigens (e.g., fusion proteins, nanodiscs, VLPs).
- Collaborate across groups to evaluate ligands for tissue-specific RNAi delivery in vitro and in vivo.
Qualifications:
- BS/MS in Biochemistry, Molecular/Cell Biology, Chemical/Biomedical Engineering, or related field with 3–5 years of biopharma experience.
- Essential: sterile cell culture technique (routine passaging, viability assays, transfection/transformation).
- Strongly desired: protein assays including ELISA, SDS-PAGE/Western blot, immunoprecipitation, SPR/BLI.
- Preferred: flow cytometry.
- Plus: molecular biology techniques (PCR, cloning, plasmid prep, NGS library prep).
- Plus: preparative scale protein purification (affinity chromatography, SEC).
- Strong organizational skills, diligent record keeping (ELN, inventory management), and ability to manage multiple projects.
- Essential: excellent written and oral communication.
- Contribute to the design and execution of ligand selection campaigns using display technologies (e.g., phage/yeast display, MACS/FACS) against diverse membrane proteins.
- Characterize ligand-receptor interactions using biochemical techniques and cell-based assays (ELISA, BLI/SPR, flow cytometry, epitope binning).
- Support library construction and hit sequence recovery using molecular biology techniques (PCR, cloning, plasmid purification).
- Assist with preparation and characterization of soluble and membrane-embedded protein antigens (e.g., fusion proteins, nanodiscs, VLPs).
- Collaborate across groups to evaluate ligands for tissue-specific RNAi delivery in vitro and in vivo.
Qualifications:
- BS/MS in Biochemistry, Molecular/Cell Biology, Chemical/Biomedical Engineering, or related field with 3–5 years of biopharma experience.
- Essential: sterile cell culture technique (routine passaging, viability assays, transfection/transformation).
- Strongly desired: protein assays including ELISA, SDS-PAGE/Western blot, immunoprecipitation, SPR/BLI.
- Preferred: flow cytometry.
- Plus: molecular biology techniques (PCR, cloning, plasmid prep, NGS library prep).
- Plus: preparative scale protein purification (affinity chromatography, SEC).
- Strong organizational skills, diligent record keeping (ELN, inventory management), and ability to manage multiple projects.
- Essential: excellent written and oral communication.