Senior Associate Scientist, Invivo Oncology Discovery

Role Summary

The Senior Associate Scientist will join the Oncology Discovery Pharmacology and In Vivo Biology group at Bristol-Myers Squibb in Cambridge, MA, to advance early-stage therapeutics in Oncology TRC. The position will utilize in vivo models to profile novel biologics such as T cell engagers and support target identification/validation through IND-enabling activities. The candidate will collaborate across Discovery Oncology, protein engineering/antibody development, DMPK and translational groups, and present results to multidisciplinary teams.

Responsibilities

• Independently conduct mouse in vivo pharmacology experiments, including model development, tumor implantation, dosing via IV, IP, SC, I-TUMOR, IM and PO routes, tumor measurements, health monitoring, tissue sampling by fine needle biopsy and core needle biopsy, peritoneal lavage, blood draws by retro-orbital, tail vessel micro-sampling, cardiac puncture and tissue/tumor collection at necropsy including fixation, freezing and tissue disaggregation to support ex vivo analyses.
• Independently conduct in vivo efficacy and PK/PD studies and experiments supporting the development of new pharmacology models.
• Contribute to the utilization of ELISAs (MSD), qPCR, or WB to monitor biomarkers and cytokines in harvested tissues & blood samples. Isolate PBMC and primary T cells from Leukopaks and functionally characterize them using cell-based assays.
• Contribute to the utilization of multi-color flow cytometry to profile the immune infiltrate in harvested mouse tissues.
• Accurately maintain proper scientific documentation associated with pharmacology studies in an electronic lab notebook.
• Effectively communicate and present summaries of research results to team members or to a multidisciplinary team of researchers.
• Rotating weekends, holidays and off-hour on-site responsibilities to support in life studies, when applicable, will be expected of this position.

Qualifications

• A B.S. with 2-7 years relevant industry or comparable academic experience and a clearly demonstrated skill set in in vivo oncology or cancer cell signaling is required.
• Demonstrated competency in independently using in vivo mouse tumor models to perform PK/PD and efficacy studies in conjunction with blood/tissue collection is required.
• Experience with subcutaneous syngeneic and xenograft tumor models is required; experience with GEMMs and PDx models is a plus.
• Competency in murine dosing, i.e. PO, IP, IV, SC, is required; intra-tumor and IM is preferred.
• Proficiency in cell culture, passaging and harvesting is required. Experience with preparing adherent and suspension mammalian cells for subcutaneous and intravenous implantation preferred.
• Experience in independently processing blood samples, harvesting and disaggregating tumors, spleen and preparing samples for FACS analysis
• Independently set up FACS multichannel panels and routinely profile tumors, blood and lymphoid organs by Flow cytometric analysis. Expected to analyze FACS data and present the analysis to the team.
• Demonstrated experience executing ex vivo assays to investigate target engagement, mechanism of action and biomarkers by qPCR, ELISA (MSD), WB. Familiar with cytokine profiling studies and analysis by MSD & Luminex methods.
• Experience with orthotopic or chemically induced mouse models or rodent surgical experience is a plus.
• Proficiency in utilizing software such as GraphPad Prism, electronic notebooks, Microsoft office etc. for data analysis and figure generation is required.
• Excellent organizational skills and a detail-oriented approach to the execution of experiments and record keeping is a requirement.
• Strong interpersonal skills with the ability to interact effectively with peers and management is required.