Scientist II, in vivo Gene Insertion

Role: In-vivo gene editing scientist focusing on developing and optimizing gene insertion techniques across various tissues and cell types, primarily utilizing CRISPR-based systems. Responsibilities: design and execute workflows involving molecular biology, primary cell handling (including T cells), and delivery methods such as transfection, viral transduction, and targeted LNPs; assess editing efficiency via ddPCR and T cell phenotyping; collaborate cross-functionally to advance projects toward clinical readiness; analyze and present data; leverage immunology expertise to innovate editing and delivery strategies; work with animal models. Requirements: PhD in Biology, Immunology, Genetics, or related field with 2-12+ years in gene editing and synthetic immunology; proven technical skills in cloning, flow cytometry, T cell culture, and genome editing assays; experience managing multiple projects; strong communication and teamwork skills. Preferred: experience with in vivo delivery modalities (tLNP, AAV, VLP), developing novel editing platforms. HighValue: focus on in vivo gene editing therapies, primarily in immunology and cell therapy for human diseases, with emphasis on translational research and clinical readiness. WorkSetup: on-site in Boston; no travel specified.