Research Associate I - Transgene Analysis

Role Summary

You're passionate about science and want to be in a hands-on role supporting our transgene analysis team. The Research Associate I will assist with basic Molecular Biology techniques and will focus on generating protein expression data from cell and tissue samples.

Responsibilities

• Apply scientific research methodologies to execute experimental protocols, troubleshoot issues, and manage daily tasks efficiently
• Perform cell and tissue homogenization and measure total protein content using a BioTEK plate reader
• Conduct and interpret SDS-PAGE Western blotting to evaluate transgene expression in cell and tissue samples
• Utilize an automated capillary western blot (Protein Simple) to assess transgene expression and serum antibody detection
• Generate quantitative western blot data using compass software and excel
• Receive, process, and inventory samples received from collaborators
• Complete western analysis of pig cell and tissue lysates, including protein quantification, sample and buffer preparation, electrophoresis and blotting, as well as detection
• Maintain inventories of porcine tissue, plasma, and serum samples (both in freezers and digital databases)
• Follow laboratory safety protocols and handle biohazardous materials, including human or animal cells and tissues
• Assist with general lab maintenance, including cleaning, autoclaving biohazardous waste, preparing buffers, and ordering supplies

Qualifications

• Required: Bachelor’s Degree in molecular biology or a related field of study
• Required: 1+ years of hands-on experience in a research lab setting
• Required: 1+ years of experience in quantitative biochemical or biological assays (e.g., DNA/protein quantification, Western blot, ELISA)
• Required: Ability to handle and process animal samples for a variety of analysis including Southern and Western analysis
• Required: Hands-on experience using Southern analysis to determine transgene presence in DNA samples
• Required: Familiarity with the concept of Western analysis
• Required: Proficient in MS Office

Skills

• Experience with protein expression analysis and Western blotting techniques
• Experience with automated capillary western systems
• Data analysis and interpretation using software tools (e.g., Compass, Excel, MS Office)
• Lab safety and biohazard handling
• Sample processing and inventory management

Education

• Bachelor’s Degree in molecular biology or a related field of study