Research Associate I-III IN VITRO

Role Summary

The IN VITRO Research Associate performs research and/or development in collaboration with others for projects. Makes detailed observations, analyzes data and interprets results. Investigates, creates and develops new methods and technologies for project advancement.

Responsibilities

• Maintain primary and established cell lines.
• Generate immunophenotyping and immune cell functional data using techniques such as multicolor flow cytometry, ELISA, and xCELLigence RTCA.
• Isolate protein, DNA, and RNA from cell cultures and tissues; perform gene and protein analysis using qPCR and Western blot.
• Support the generation and purification of recombinant viruses. Perform VPA assay.
• Assist with general cell culture for the production of oncolytic viruses, including herpes simplex virus, vaccinia virus, and/or adenovirus.
• Analyze and organize experimental data with strong attention to detail.
• Assist with laboratory maintenance, inventory, and cleaning tasks.
• Adhere to EH&S guidelines, participate in mandatory safety training, and follow SOPs to ensure study integrity and compliance.
• Support the in vivo team as needed, including animal handling and sample collection.
• Perform other duties as assigned.

Qualifications

• 3+ years of experience in a biotechnology company with broad knowledge and experience in cell and molecular biology methods; Immuno-Oncology field is preferred.
• Experience with mammalian cell culture is required.
• Experience in a range of in vitro cellular/molecular biology techniques (cell culture, cloning, qPCR, Western blot, immunohistochemistry, microscopy).
• Experience in immune cell based assays (ELISA, MSD, cytotoxicity assays). Strong hands-on experience in acquiring and analyzing flow cytometry data (multi-parameter flow cytometry, intracellular, extracellular staining, FlowJo analysis) is highly desirable.
• Experience with mouse handling, including restraint, injection (IP, IV, SC), and tissue collection is highly desirable.
• Excellent data organization, analysis, and troubleshooting skills using electronic lab notebook.
• Dedication to working in a team-oriented environment to contribute to shared project.
• Strong work ethic, self-motivation, and ability to multi-task in a fast-paced work environment.
• Strong knowledge of MS Word, Outlook, PowerPoint, Excel, GraphPad Prism, FlowJo.

Education

• B.S. or higher in life science, or related field

Additional Requirements

• Mathematical Skills:
  • Ability to work with mathematical concepts such as calculation of dilutions and statistical analyses.
  • Ability to apply concepts such as fractions, percentages, ratios, and proportions to practical situations.
• Reasoning Ability:
  • Ability to define problems, collect data, establish facts, and draw valid conclusions.
• Language Skills:
  • Excellent communication skills with ability to effectively communicate with other research teams
  • Ability to read and understand technical procedures
  • Ability to write protocols and reports
  • Ability to effectively present data and respond to questions from co-workers