Principal Associate Scientist, Target Validation
Alnylam Pharmaceuticals
August 07, 2026
On-site
Cambridge, MA
Clinical Research and Development
Onsite role primarily located in Cambridge, MA.
Key Responsibilities:
- Conduct mechanistic target validation and proof-of-concept preclinical research across tissues/therapeutic areas.
- Perform in vivo proof-of-concept studies evaluating novel extrahepatic targeted siRNA delivery platforms across programs.
- Develop, optimize, and validate assays for emerging targets, biomarker development, and tissue-specific delivery evaluation.
- Integrate knockdown data with orthogonal readouts (protein, functional, and transcriptomic endpoints) to support internal decisions.
- Present experimental reports/data to diverse audiences.
- Maintain accurate laboratory notebooks and electronic data records with data integrity.
Qualifications:
- BS/MS in Biology/Cell/Molecular Biology (or related) with 5–8 years’ experience (preferably industrial).
- Experience with in vivo rodent studies: subcutaneous, IP/IV dosing, retro-orbital bleeding, and tissue dissections.
- RNA extraction across tissue types; RNA/DNA/protein quantification assays (qPCR, Western blotting, ELISA, immunoprecipitations).
- Mammalian cell culture experience (maintenance, transfection) including primary cells (e.g., hepatocytes, cardiomyocytes, muscle cells).
- Quantitative analysis skills (GraphPad Prism, Excel, statistics) and meticulous lab notebook documentation.
- Preferred: muscle/cardiovascular/endocrine disease biology and relevant models.
- Ability to independently perform/troubleshoot assays; strong written and oral communication.
Pay & Benefits (as stated): $95,600–$143,400 base; eligible for annual short-term incentive and long-term incentive (e.g., equity). Medical/dental/vision, life/disability, lifestyle reimbursement, FSAs/HSAs, 401(k) match, paid time off, wellness days, holidays, recharge breaks, family resources and leave.
Key Responsibilities:
- Conduct mechanistic target validation and proof-of-concept preclinical research across tissues/therapeutic areas.
- Perform in vivo proof-of-concept studies evaluating novel extrahepatic targeted siRNA delivery platforms across programs.
- Develop, optimize, and validate assays for emerging targets, biomarker development, and tissue-specific delivery evaluation.
- Integrate knockdown data with orthogonal readouts (protein, functional, and transcriptomic endpoints) to support internal decisions.
- Present experimental reports/data to diverse audiences.
- Maintain accurate laboratory notebooks and electronic data records with data integrity.
Qualifications:
- BS/MS in Biology/Cell/Molecular Biology (or related) with 5–8 years’ experience (preferably industrial).
- Experience with in vivo rodent studies: subcutaneous, IP/IV dosing, retro-orbital bleeding, and tissue dissections.
- RNA extraction across tissue types; RNA/DNA/protein quantification assays (qPCR, Western blotting, ELISA, immunoprecipitations).
- Mammalian cell culture experience (maintenance, transfection) including primary cells (e.g., hepatocytes, cardiomyocytes, muscle cells).
- Quantitative analysis skills (GraphPad Prism, Excel, statistics) and meticulous lab notebook documentation.
- Preferred: muscle/cardiovascular/endocrine disease biology and relevant models.
- Ability to independently perform/troubleshoot assays; strong written and oral communication.
Pay & Benefits (as stated): $95,600–$143,400 base; eligible for annual short-term incentive and long-term incentive (e.g., equity). Medical/dental/vision, life/disability, lifestyle reimbursement, FSAs/HSAs, 401(k) match, paid time off, wellness days, holidays, recharge breaks, family resources and leave.