Principal Associate Scientist, RNAi Discovery

Role Summary

Principal Associate Scientist, RNAi Discovery — Onsite, Cambridge, MA. Lead high-throughput in vitro RNAi research within the RNAi Discovery group, driving target identification through lead candidate selection.

Responsibilities

• Conduct a wide range of in vitro siRNA research studies to evaluate RNAi compounds using biochemical, molecular, and cell-based assays.
• Plan, coordinate, and execute day-to-day high-throughput in vitro experiments, including transfection, RNA extraction, qPCR, data upload, and statistical analysis.
• Design, perform, and interpret experiments independently and collaboratively, providing critical troubleshooting and technical insight.
• Maintain and culture various cell types (iPSCs, primary cells, immortalized cell lines) to support research objectives and facilitate high-throughput siRNA screening through qPCR and cell-based assays.
• Ensure accurate data recording, analysis, and timely reporting, preparing clear and detailed experimental summaries, documentation, and presentations.
• Identify and resolve technical challenges, contributing to the ongoing optimization and development of experimental protocols.
• Operate and maintain automated instrumentation and robotic systems to support high-throughput (384-well) screening workflows.

Qualifications

• BS/MS in Biology, Molecular/Cell Biology, Biochemistry, or related field with 5–8 years of relevant experience, preferably in an industrial or biotech environment
• Proficiency in mammalian cell culture, including maintenance and transfection of immortalized and primary cell lines
• Skilled in molecular biology techniques for RNA, DNA, and protein quantification (qPCR, dPCR, Western blotting, ELISA, immunoprecipitation)
• Prior experience or strong understanding of nucleic acid–based therapeutics (siRNA, ASO, CRISPR) highly desired
• Hands-on experience with laboratory automation systems, including liquid-handling platforms such as PerkinElmer JANUS, Beckman i7, or similar
• Strong knowledge of experimental design, data interpretation, and scientific troubleshooting
• Highly detail-oriented, organized, and capable of managing multiple projects in a fast-paced, dynamic environment
• Demonstrated ability to collaborate and communicate effectively with cross-functional teams and stakeholders
• Proficiency with Microsoft Office (Excel, Outlook, PowerPoint, Word) and GraphPad Prism

Skills

• In vitro RNAi assay design and analysis
• High-throughput screening techniques
• Cell culture and transfection of mammalian cells
• qPCR, dPCR, protein quantification methods
• Data interpretation and troubleshooting
• Laboratory automation and robotic systems

Education

• BS/MS in Biology, Molecular/Cell Biology, Biochemistry, or related field