Molecular Biology Scientist/Senior Associate Scientist
Legend Biotech
August 06, 2026
On-site
Philadelphia, PA
Clinical Research and Development
Molecular Biology Scientist/Senior Associate Scientist (Vector Biology) — Philadelphia, PA
Key Responsibilities:
- Independently execute lentiviral vector plasmid/helper plasmid design and construction for gene overexpression, knock-down, and knock-out.
- Perform molecular cloning workflows; ensure plasmid QC for vector production.
- Maintain accurate plasmid records (sequence files, maps, inventory/locations) and support construct requests.
- Generate and characterize engineered mammalian cell lines (transient/stable vector production, single-cell cloning, cell engineering, basic characterization).
- Perform mammalian cell culture and transient transfection under BSL-2.
- Support small-scale vector production using suspension cell lines.
- Evaluate vector performance using qPCR/dPCR, ELISA/Western blot, and flow cytometry.
- Analyze data, present conclusions, escalate issues, and recommend next steps.
- Maintain compliant electronic lab notebooks and documentation; adhere to BSL-2 biosafety standards.
- Support cross-functional tech transfer as needed; plan day-to-day execution and recommend optimizations.
Requirements:
- Scientist: M.S. with 5+ years or Ph.D. with 0–2 years; hands-on molecular cloning, cell engineering, and mammalian cell culture.
- Hands-on cloning/plasmid prep/sequence analysis and construct verification.
- Mammalian cell culture and transient transfection; BSL-2 practices/documentation.
- Engineered cell line generation (stable expression and/or CRISPR KO/KI) with cloning and basic screening.
- Exposure to viral vector workflows (LVV preferred) and titering/functional readouts.
- Experience with qPCR/dPCR, flow cytometry, Western blot, ELISA.
- Strong ELN/documentation, sample tracking, SOP adherence, and clear communication.
Benefits (as stated): Medical/dental/vision; 401(k) with company match; 8 weeks paid parental leave after 3 months; paid time off (vacation, personal, sick, floating holidays) plus 11 company holidays. Performance-based bonus and/or equity (eligible roles).
Pay Range (base): $93,463–$122,670 USD.
Key Responsibilities:
- Independently execute lentiviral vector plasmid/helper plasmid design and construction for gene overexpression, knock-down, and knock-out.
- Perform molecular cloning workflows; ensure plasmid QC for vector production.
- Maintain accurate plasmid records (sequence files, maps, inventory/locations) and support construct requests.
- Generate and characterize engineered mammalian cell lines (transient/stable vector production, single-cell cloning, cell engineering, basic characterization).
- Perform mammalian cell culture and transient transfection under BSL-2.
- Support small-scale vector production using suspension cell lines.
- Evaluate vector performance using qPCR/dPCR, ELISA/Western blot, and flow cytometry.
- Analyze data, present conclusions, escalate issues, and recommend next steps.
- Maintain compliant electronic lab notebooks and documentation; adhere to BSL-2 biosafety standards.
- Support cross-functional tech transfer as needed; plan day-to-day execution and recommend optimizations.
Requirements:
- Scientist: M.S. with 5+ years or Ph.D. with 0–2 years; hands-on molecular cloning, cell engineering, and mammalian cell culture.
- Hands-on cloning/plasmid prep/sequence analysis and construct verification.
- Mammalian cell culture and transient transfection; BSL-2 practices/documentation.
- Engineered cell line generation (stable expression and/or CRISPR KO/KI) with cloning and basic screening.
- Exposure to viral vector workflows (LVV preferred) and titering/functional readouts.
- Experience with qPCR/dPCR, flow cytometry, Western blot, ELISA.
- Strong ELN/documentation, sample tracking, SOP adherence, and clear communication.
Benefits (as stated): Medical/dental/vision; 401(k) with company match; 8 weeks paid parental leave after 3 months; paid time off (vacation, personal, sick, floating holidays) plus 11 company holidays. Performance-based bonus and/or equity (eligible roles).
Pay Range (base): $93,463–$122,670 USD.