Role Responsibilities:
- Develop novel culture systems and in vitro assays to characterize innate immune responses to next-generation vaccines (immune cell processing, cytokine quantification, flow cytometry, immunofluorescence, gene expression).
- Support mechanism of action (MoA) studies.
- Apply functional molecular biology techniques: independently clone novel constructs; perform DNA/RNA isolation and transfections; quantify RNA via RT-qPCR and/or NGS.
- Conduct and troubleshoot experiments; interpret, analyze, and summarize data with supervision.
- Present data in group and project team meetings and to the department as required.
- Maintain detailed laboratory notebook (ELN) records in compliance with company policy.
- Execute qualifications/validations of new instruments and custom methods.
Basic Qualifications:
- BS (1β3 years relevant experience) in biology, microbiology, immunology, chemistry, chemical engineering, or related field.
- Experience with cellular and molecular biology techniques: flow cytometry, confocal microscopy-based assays, RT-qPCR, Luminex/MSD, molecular cloning, cell culture, DNA/RNA isolation, in-vitro transcription, DNA/RNA transfection.
- Proficient with Microsoft Office (Excel, Word, PowerPoint).
- Attention to detail; accurate recordkeeping and adherence to SOPs.
- Curiosity and willingness to learn additional techniques.
- Excellent written and oral communication skills; team player.
Preferred Qualifications:
- MS (0β2 years relevant experience) in virology/microbiology/immunology/biology/chemistry/chemical engineering or related field.
- Experience with complex culture systems (3D-cultures, co-cultures, ex-vivo, immune cells).
- GraphPad (PRISM) data analysis/plotting.
- Immunostaining assay experience (flow, IF, MSD/Luminex).
- Sequence analysis software proficiency (Geneious/Sequencher/DNAStar).
- Experience designing and running experiments independently.