Associate Scientist II/Scientist I (Biologics Expression and Purification)
MacroGenics, Inc.
Full-time
On-site
Rockville, MD
$67,200 - $128,800 USD yearly
Clinical Research and Development
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Role Summary
Associate Scientist II/Scientist I (Biologics Expression and Purification) - As a member of the Protein Science team, this individual will set up transfections to express proteins in mammalian cells followed by their purification and characterization to support the Biologics discovery pipeline.
Responsibilities
- Culture of mammalian cells in suspension following aseptic techniques and cell-line specific protocols.
- Transient transfection of cell lines or expansion of stable cell pools, from 3 ml – 10 L scale, to express proteins of interest. Monitor and optimize culture conditions to maximize protein quality and expression.
- Protein purification from milligram to gram scale using semi-automated FPLC systems.
- Analytical characterization of purified proteins to support research projects for drug discovery.
- Collect, analyze, and summarize experimental data in electronic lab notebook for archiving and sharing.
- Maintain lab and lab equipment in optimal functioning condition. Report any problems to supervisor.
Qualifications
- Bachelor’s degree in Biology or Biochemistry or a related field of study with a minimum of 2 years of bench-related experience (Associate Scientist II).
- Bachelor’s degree in Biology or Biochemistry or a related field of study with a minimum of 3 years of bench-related experience (Scientist I).
- Or Master’s degree in Biology or Biochemistry or a related field of study with a minimum of 1 year of bench-related experience (strongly preferred).
Knowledge, Skills And Abilities
- Experience with mammalian cell culture, transfections, maintaining cell lines, etc. with meticulous sterile technique and lab practices.
- Experience with recombinant protein purification and analytical characterization (CE or SDS-PAGE, SEC).
- Experience with experimental design and execution with minimal supervision.
- Experience with standard lab software for data analysis (MS Excel, Prism, etc.). Good organizational skills and ability to multi-task.
- Good verbal and written communication skills. Demonstrated ability to work in a collaborative environment with other team members.
- Willingness to work on occasional weekends.
Preferred Experience
- Experience with antibody purification using automated FPLC system.
- Experience with quantitative assays (BLI Octet, ELISA, etc.).