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Associate Scientist, HTS & NGS

KorroBio
2 months ago
Full-time
On-site
60 First St, Cambridge, MA 02141
Clinical Research and Development

Key Responsibilities:

  • ​​​Independently design and execute high-throughput cell-based assays to support oligonucleotide platform and portfolio projects  
  • Perform amplicon-seq library preparation and quality control, operate and maintain NGS platforms and associated laboratory equipment 
  • ​Troubleshoot HTS assays, library preparation, and sequencing workflows as needed 
  • ​Analyze and present HTS-NGS data based on results in a team setting; collaborate with cross-functional teams to support lead discovery 
  • ​Support NGS scientists in operational tasks, including inventory management, assay development, and operations improvement 
  • ​Accurately document project and sample information, protocols, and experimental details through an Electronic Lab Notebook (ELN) 

Required Qualifications:

  • ​​​MS with 3+ years or BS with 5+ years of experience in molecular biology, cell biology, or related field 
  • ​1+ years of hands-on experience with high-throughput assays and automated workflows 
  • ​Experience with cell culture techniques, including plating and transfection in 96/384-well formats 
  • ​Experience with NGS assays: amplicon-seq and RNA-seq, including primer design, library preparation, quantification, and data quality control 
  • ​Demonstrated expertise in molecular biology techniques: RNA/DNA isolation, transfection, PCR/qPCR, gel electrophoresis
  • ​Familiarity with liquid handling systems (e.g., Viaflo96/384, HiRes/Certus Flex, Tempest) is highly preferred  
  • ​Hands-on experience with next-generation sequencing platforms and equipment including Illumina (MiSeq i100), Qubit, Tape Station, and Fragment Analyzer is highly preferred 
  • ​Experience with LIMS systems (Benchling) and project management tools (Jira/Confluence)​​ 
  • ​Strong troubleshooting skills and ability to suggest and implement new solutions independently 
  • ​Excellent written and verbal communication skills with attention to detail 
  • ​Ability to thrive in a fast-paced and evolving environment ​​