Advisor / Sr. Advisor, Cell Engineering - CAR Biology - LGT
Eli Lilly and Company
6 hours ago
On-site
Boston, MA
Clinical Research and Development
Responsibilities:
- Design, implement, and optimize delivery of transposable element constructs and other genetic payloads into primary human cells (including HSCs) using non-viral platforms; engineer and iterate DNA payload architectures to optimize integration efficiency, expression durability, and cytotoxicity
- Develop and validate analytical assays to characterize integration outcomes, genomic insertion site distributions, and long-term transgene expression stability in primary cells
- Support cell engineering functional workflows with the broader team, including viability assays and flow-based phenotyping to assess delivery and integration outcomes
- Translate ex vivo delivery findings to inform in vivo delivery strategy; partner with formulation teams on co-delivery of DNA and RNA payloads
- Mentor junior scientists; serve as the primary technical resource for transgene delivery and integration across the team
Basic Requirements:
- PhD in Molecular Biology, Cell Biology, Biochemistry, Bioengineering, or closely related field
- 3+ years of postdoctoral or industry experience
Additional Preferences:
- Experience with transposon-based integration systems (Sleeping Beauty, PiggyBac, or equivalent) in primary immune cells, including payload design and ITR configuration
- Hands-on experience delivering DNA constructs into primary human T cells and/or HSCs
- Proficiency in integration analytics: ddPCR VCN, integration site profiling, and transgene expression characterization
- Strong molecular biology foundation: cloning; minicircle or plasmid design
- Experience with LNP or other nanoparticle platforms for nucleic acid delivery
- Experience in high-throughput cloning or screening
Benefits (as explicitly listed):
- Eligibility for company bonus (depending on company and individual performance)
- Comprehensive benefits for eligible employees: 401(k), pension, vacation; medical/dental/vision/prescription drug; flexible benefits (healthcare/dependent day care); life insurance/death benefits; time off and leave of absence; well-being benefits (EAP, fitness, employee clubs/activities)
- Design, implement, and optimize delivery of transposable element constructs and other genetic payloads into primary human cells (including HSCs) using non-viral platforms; engineer and iterate DNA payload architectures to optimize integration efficiency, expression durability, and cytotoxicity
- Develop and validate analytical assays to characterize integration outcomes, genomic insertion site distributions, and long-term transgene expression stability in primary cells
- Support cell engineering functional workflows with the broader team, including viability assays and flow-based phenotyping to assess delivery and integration outcomes
- Translate ex vivo delivery findings to inform in vivo delivery strategy; partner with formulation teams on co-delivery of DNA and RNA payloads
- Mentor junior scientists; serve as the primary technical resource for transgene delivery and integration across the team
Basic Requirements:
- PhD in Molecular Biology, Cell Biology, Biochemistry, Bioengineering, or closely related field
- 3+ years of postdoctoral or industry experience
Additional Preferences:
- Experience with transposon-based integration systems (Sleeping Beauty, PiggyBac, or equivalent) in primary immune cells, including payload design and ITR configuration
- Hands-on experience delivering DNA constructs into primary human T cells and/or HSCs
- Proficiency in integration analytics: ddPCR VCN, integration site profiling, and transgene expression characterization
- Strong molecular biology foundation: cloning; minicircle or plasmid design
- Experience with LNP or other nanoparticle platforms for nucleic acid delivery
- Experience in high-throughput cloning or screening
Benefits (as explicitly listed):
- Eligibility for company bonus (depending on company and individual performance)
- Comprehensive benefits for eligible employees: 401(k), pension, vacation; medical/dental/vision/prescription drug; flexible benefits (healthcare/dependent day care); life insurance/death benefits; time off and leave of absence; well-being benefits (EAP, fitness, employee clubs/activities)